technology

Dyadic is using the fungi Trichoderma longibrachiatum, Aspergillus foetidus and Myceliophthora thermophila to produce its enzymes.

The fungal C1 expression system has been developed and patented by Dyadic. This C1 strain is unique because it displays two distinct morphological forms; as hyphae and small hyphal fragments which are called propagules. This feature renders C1 a so called low-viscosity phenotype which allows the use of the fungus in a fermentation process that is not hampered by high viscosity conditions. In addition the low-viscosity phenotype allows for easy liquid handling of C1, which is particularly useful in High Troughput Robotic screening.

Using the C1 expression system high production levels (extracellular) of a variety of functional heterologous and homologous proteins and enzymes have been achieved. Examples of such proteins are: full length (heterodimeric) human antibodies and specific enzymes for feed, food and paper & pulp applications. In addition strains are constructed that in one fermentation produce an enzyme mixture tailored to the needs of the application process.

Characteristics of the C1 system are:

  • The C1 fermentation process is robust and easy to scale up from 1L to 50.000L
  • The costs of proteins produced by C1 are relatively low due to the high productivity, the low viscosity during fermentation and the short cycle times
  • High protein yields (up to 100 g/L) of extracellular enzymes can be obtained
  • The genome has been sequenced and to a large extend annotated
  • A broad range of strains over-expressing single C1-enzymes have been developed
  • C1 has shown to be is an excellent host for the production of heterologous proteins
  • Versatile genetic tools (expression vectors) are available
  • A broad range of C1-host strains (e.g. low-protease hosts) are available
  • C1 Safety profile: self affirmed GRAS
  • Owned and patented by one party (Dyadic)


disclaimer - Dyadic International