technology

Dyadic is using the fungi Trichoderma longibrachiatum, Aspergillus foetidus and Chrysosporium lucknowense (C1) to produce its enzymes.

The fungal C1 expression system has been developed and patented by Dyadic. This C1 strain is unique because it displays two distinct morphological forms; as hyphae and small fragments which are called propagules. This feature of C1 allows the use of the fungus in a High Throughput Robotic System for the identification of genes of interest followed by the over-expression of the gene in a fermentation process that is not hampered by high viscosity conditions.


Characteristics of the C1 system are:

  • High protein yields (up to 100 g/L) of extracellular enzymes can be obtained
  • The genome has been sequenced and to a large extend annotated
  • A broad range of strains over-expressing single C1-enzymes have been developed
  • Versatile genetic tools (expression vectors) are available
  • Mutants and transformants, both low in protease activity, are available
  • The C1 fermentation process is robust and easy to scale up from 1L to 50.000L
  • The costs of proteins produced by C1 are relatively low due to the high productivity, the low viscosity during fermentation and the short cycle times
  • C1 can be used to identify a gene in a High Throughput Screening approach followed by the over-expression of this gene
  • C1 Safety profile: self affirmed GRAS
  • Owned and patented by one party (Dyadic)

 

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