antibodies

The power of the C1 expression system was demonstrated by the production of functional full length human monoclonal antibodies using highly productive low protease mutant Chrysosporium host strains.

High level expression was achieved using a glucoamylase- carrier approach, and recombinant strains expressing both heavy and light chains were obtained. Heterodimeric antibody molecules were formed efficiently, allowing simple purification of the protein from the culture fluid using Protein A.

Cell-based bio-assays performed on the culture supernatant and the purified samples revealed almost complete bioactivity and production of improved antibodies.

For more information see: Production of full length antibody chains in Chrysosporium